Cell Cycle Analysis

Cell cycle analysis involves using flow cytometry so as to analyse the toxicity of the anti-cancer treatment through the number of cells in each cell cycle. The process is done by first seeding the cells overnight and treated with various kinds of treatments such as anticancer drug, doxorubicin. The cells are then trypsinised from the wells and harvested by centrifugation. Resuspension of the cells is conducted and the cells are fixed with fixing solution, then stored at -20 ºC overnight. The next day, the fixing solution is removed by centrifugation and then decanting of the solution. The cells are then stained with the staining solution for 1 hour, so that will be detected by the flow cytometer. The samples are then measured at low flow rate at an excitation wavelength of 488nm and emission of orange/red fluorescent. Cells at sub G1 phase indicate death. So the higher the number of cells at the sub G1 phase, the more toxic is the anticancer treatments.


This above shows how the flow cytometer presents the results after reading the various samples treated by the various treatments such as the anticancer drug, doxorubicin.

Gwendolynn Tan
TG02
0703953J